Cloning, expression, purification and characterization of Plasmodium spp. glyceraldehyde-3-phosphate dehydrogenase
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- 作者：Prakash B. Sangolgi ; Chinthapalli Balaji ; Sneha Dutta ; Nitin Jindal ; Gotam K. Jarori ; gkjarori@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：2DGE ; 2-dimensional gel electrophoresis ; DDF ; differential detergent fractionation ; Gapdh ; glyceraldehyde-3-phosphate dehydrogenase ; rPfGapdh ; recombinant Plasmodium falciparum glyceraldehyde-3-phosphate dehydrogenase ; PyGapdh ; P. yoelii Gapdh ; RmGapdh ; rabbit muscle Gapdh ; G-3-P ; d-glyceraldehyde-3-phopshate ; PTM ; post translational modification ; PBST ; phosphate buffer saline Tween ; FITC ; fluorescein isothiocyanate ; PVDF ; polyvinylidene fluoride ; RBCs ; red blood cells
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：17-25
- 全文大小：1688 K
文摘
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Association of GAPDH with multiple organelles was observed in
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1D and 2D-gel electrophoretic profiles indicate that there are multiple molecular species of PyGapdh.
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Diverse distribution and multiple PTMs suggest moonlighting functions for this glycolytic enzyme.