Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies

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• 作者：Xiaolu Yang¹ ; ² ; ⁴ ; Jinqiang Nian¹ ; ⁴ ; Qingjun Xie¹ ; ² ; Jian Feng¹ ; ² ; Fengxia Zhang¹ ; Hongwei Jing¹ ; ² ; Jian Zhang¹ ; Guojun Dong³ ; Yan Liang¹ ; Juli Peng¹ ; Guodong Wang¹ ; Qian Qian³ ; Jianru Zuo¹ ; ^{jrzuo@genetics.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：rice ; Fd-GOGAT ; nitrogen assimilation ; carbon&ndash ; nitrogen balance ; genetic variations
• 刊名：Molecular Plant
• 出版年：2016
• 出版时间：7 November 2016
• 年：2016
• 卷：9
• 期：11
• 页码：1520-1534
• 全文大小：3415 K

文摘

Plants assimilate inorganic nitrogen absorbed from soil into organic forms as Gln and Glu through the glutamine synthetase/glutamine:2-oxoglutarate amidotransferase (GS/GOGAT) cycle. Whereas GS catalyzes the formation of Gln from Glu and ammonia, GOGAT catalyzes the transfer of an amide group from Gln to 2-oxoglutarate to produce two molecules of Glu. However, the regulatory role of the GS/GOGAT cycle in the carbon–nitrogen balance is not well understood. Here, we report the functional characterization of rice ABNORMAL CYTOKININ RESPONSE 1 (ABC1) gene that encodes a ferredoxin-dependent (Fd)-GOGAT. The weak mutant allele abc1-1 mutant shows a typical nitrogen-deficient syndrome, whereas the T-DNA insertional mutant abc1-2 is seedling lethal. Metabolomics analysis revealed the accumulation of an excessive amount of amino acids with high N/C ratio (Gln and Asn) and several intermediates in the tricarboxylic acid cycle in abc1-1, suggesting that ABC1 plays a critical role in nitrogen assimilation and carbon–nitrogen balance. Five non-synonymous single-nucleotide polymorphisms were identified in the ABC1 coding region and characterized as three distinct haplotypes, which have been highly and specifically differentiated between japonica and indica subspecies. Collectively, these results suggest that ABC1/OsFd-GOGAT is essential for plant growth and development by modulating nitrogen assimilation and the carbon–nitrogen balance.