This is the first study that Cohn Fraction IV was used to purify α2-macroglobulin, which makes better use of human plasma.
Yield of 45% and purity of 95% were achieved simultaneously regardless of haptoglobin type.
Simple process of salting-out method and immobilized metal affinity chromatography facilitate pilot scale preparation.
α2-M could be readily inactivated on the condition of temperature above 50 °C, pH above 9.0 or below 4.0, and the existence of methylamine.
This is the first study that Cohn Fraction IV was used to purify α2-macroglobulin, which makes better use of human plasma.
Yield of 45% and purity of 95% were achieved simultaneously regardless of haptoglobin type.
Simple process of salting-out method and immobilized metal affinity chromatography facilitate pilot scale preparation.
α2-M could be readily inactivated on the condition of temperature above 50 °C, pH above 9.0 or below 4.0, and the existence of methylamine.