The role of CD11b in phagocytosis and dendritic cell development

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• 作者：Jingtao Chen ; Sahori Namiki ; Makiko Toma-Hirano ; Shoichiro Miyatake ; Koji Ishida ; Yasue Shibata ; Naoko Arai ; Ken-ichi Arai ; Yumiko Kamogawa-Schifter
• 关键词：DC ; Phagocytosis ; Integrin ; Antigen presentation ; Apoptosis
• 刊名：Immunology Letters
• 出版年：2008
• 出版时间：30 September 2008
• 年：2008
• 卷：120
• 期：1-2
• 页码：42-48
• 全文大小：822 K

文摘

Activation of resting T cells is highly dependent on dendritic cells (DCs), which take up antigens and present antigenic peptides to T cells in the context of the major histocompatibility complex (MHC). In this study, we generated a monoclonal antibody, which we call 1C4 that recognizes integrin α_Mβ₂ (CD11b/CD18) on the surface of conventional DCs (cDCs) and is internalized after binding. Addition of 1C4 inhibited the ability of immature DCs to phagocytose apoptotic cells. 1C4 treatment also partially inhibited the generation of cDCs from bone marrow in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF). Our findings suggest that not only CD11b is involved in the phagocytosis of apoptotic cells, but also that mAb such as 1C4 may be a useful tool for the delivery of specific proteins into the cytoplasm of immature DCs.