Evaluation of FTIR spectroscopy as diagnostic tool for colorectal cancer using spectral analysis

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• 作者：Liu Dong ; Xuejun Sun ; Zhang Chao ; Shiyun Zhang ; Jianbao Zheng ; Rajendra Gurung ; Junkai Du ; Jingsen Shi ; Yizhuang Xu ; Yuanfu Zhang ; Jinguang Wu
• 关键词：FTIR spectroscopy ; Colorectal cancerl ; Principal component analysis ; Spectrum
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：25 March, 2014
• 年：2014
• 卷：122
• 期：Complete
• 页码：288-294
• 全文大小：1377 K

文摘

The aim of this study is to confirm FTIR spectroscopy as a diagnostic tool for colorectal cancer. 180 freshly removed colorectal samples were collected from 90 patients for spectrum analysis. The ratios of spectral intensity and relative intensity (/I1460) were calculated. Principal component analysis (PCA) and Fisher鈥檚 discriminant analysis (FDA) were applied to distinguish the malignant from normal. The FTIR parameters of colorectal cancer and normal tissues were distinguished due to the contents or configurations of nucleic acids, proteins, lipids and carbohydrates. Related to nitrogen containing, water, protein and nucleic acid were increased significantly in the malignant group. Six parameters were selected as independent factors to perform discriminant functions. The sensitivity for FTIR in diagnosing colorectal cancer was 96.6% by discriminant analysis. Our study demonstrates that FTIR can be a useful technique for detection of colorectal cancer and may be applied in clinical colorectal cancer diagnosis.