Spleen injury and apoptotic pathway in mice caused by titanium dioxide nanoparticules

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• 作者：Na Li ; Yanmei Duan ; Mengmeng Hong ; Lei Zheng ; Min Fei ; Xiaoyang Zhao ; Jue Wang ; Yaling Cui ; Huiting Liu ; Jingwei Cai ; Songjie Gong ; Han Wang ; Fashui Hong
• 关键词：Titanium dioxide nanoparticules ; Mice ; Spleen ; Apoptotic pathway ; Oxidative stress
• 刊名：Toxicology Letters
• 出版年：2010
• 出版时间：2 June 2010
• 年：2010
• 卷：195
• 期：2-3
• 页码：161-168
• 全文大小：1217 K

文摘

Nanoparticulate titanium dioxide (TiO₂) has been demonstrated to decrease immunity of mice, but very little is known about the injury of spleen involved immunomodulation and its molecular mechanism. In order to understand the spleen injury induced by intraperitoneal injection of TiO₂ nanoparticules (NPs) for consecutive 45 days, the spleen pathological changes, apoptosis, the expression levels of the apoptotic genes and their proteins, and oxidative stress in the mouse spleen were investigated. The results demonstrated that TiO₂ NPs had obvious accumulation in the mouse spleen, leading to congestion and lymph nodule proliferation of spleen tissue, and splenocyte apoptosis. TiO₂ NPs effectively activated caspase-3 and -9, decreased the Bcl-2 the levels of gene and protein, and increase the levels of Bax, and cytochrome c genes and their protein expression, promoted ROS accumulation. Taken together, this study indicated that TiO₂ NPs-induced apoptosis in the mouse splenocyte via mitochondrial-mediated pathway. These findings provide strong evidence that the TiO₂ NPs can induce the spleen pathological changes, apoptosis, leading to the reduction of immunity of mice.