A chiral LC-MS/MS method for the stereospecific determination of efonidipine in human plasma
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- 作者：Man Liu ; Ming Deng ; Dan Zhang ; Xiaolin Wang ; Jingyi Ma ; Hongna Zhao ; Lina Zhang ; Yang Tong ; Huichen Liu ; huichenliu@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：LLE ; liquid-liquid extraction ; DHP ; dihydropyridine ; CKD ; chronic kidney disease ; BP ; blood pressure ; MBF ; medullary blood flux ; IS ; internal standard ; ESI ; electrospray ionization ; CAD ; collision activated dissociation ; EP ; entrance potential ; CXP ; collision cell exit potential ; DP ; declustering potential ; MRM ; multiple reaction monitoring ; CE ; collision energy ; QC ; quality control ; LLOQ ; lower limit of quantification ; ULOQ ; upper limit of quantification ; RSD ; relative standard deviation ; RE ; r
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：122
- 期：Complete
- 页码：35-41
- 全文大小：872 K
文摘
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The first chiral method for quantification of efonidipine enantiomers in human plasma was developed.
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The method utilized a chiral stationary phase column, one-step LLE, and good sensitivity.
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The method has been validated following bioanalytical validation guidelines.
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The method was applied to the PK of efonidipine enantiomers in human subject for the first time.