A viral satellite DNA vector-induced transcriptional gene silencing via DNA methylation of gene promoter in Nicotiana benthamiana
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- 作者：Zheng Jua ; zhengju2016@126.com" class="auth_mail" title="E-mail the corresponding author ; Lei Wanga ; lwang2015@126.com" class="auth_mail" title="E-mail the corresponding author ; Dongyan Caoa ; caody2012@126.com" class="auth_mail" title="E-mail the corresponding author ; Jinhua Zuob ; zuojinhua@126.com" class="auth_mail" title="E-mail the corresponding author ; Hongliang Zhua ; hlzhu@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Daqi Fua ; daqifu@126.com" class="auth_mail" title="E-mail the corresponding author ; Yunbo Luoa ; lyb@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Benzhong Zhua ; zbz@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：ADK ; adenosine kinase ; AGO ; ARGONAUTE ; ALSV ; apple latent spherical virus ; BCTV ; beet curly top virus ; BSCTV ; beet severe curly top virus ; CaLCuV ; cabbage leaf curl virus ; CaMV ; cauliflower mosaic virus ; CLCuKoV ; cotton leaf curl Kokhran virus ; CLCuMuB ; cotton leaf curl Multan beta-satellite ; CMV ; cucumber mosaic virus ; DCL ; DICER-LIKE ; dpi ; days post inoculation ; dsRNAs ; double-stranded RNAs ; GFP ; green fluorescent protein ; MCS ; multiple cloning site ; MYMIV ; mungbean yellow mosaic India virus
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 September 2016
- 年：2016
- 卷：223
- 期：Complete
- 页码：99-107
- 全文大小：1986 K
文摘
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A viral satellite DNA was integrated to a more efficient p1.7A + 2mβ vector.
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The TYLCCNV:PDS vector efficiently silenced PDS gene in N. benthamiana plants.
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The TYLCCNV:35S vector obviously silenced GFP gene in N. benthamiana (16c) plants.
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GFP silencing induced by TYLCCNV:35S was heritable in N. benthamiana (16c) plants.
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Highly methylated cytosine of 35S promoter in TYLCCNV:35S-inoculated plants (16c).