Efficient protein digestion using highly-stable and reproducible trypsin coatings on magnetic nanofibers
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- 作者：Byoungsoo Leea ; 1 ; Byoung Chan Kimb ; 1 ; Mun Seock Changc ; Han Sol Kima ; Hyon Bin Nad ; Yong Il Parke ; Jinwoo Leef ; Taeghwan Hyeone ; Hookeun Leeg ; Sang-Won Leec ; sw_lee@korea.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Jungbae Kima ; jbkim3@korea.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：TR ; trypsin ; CT ; α-chymotrypsin ; GA ; glutaraldehyde ; THF ; tetrahydrofuran ; BAPNA ; Nα-benzoyl-l-arginine 4-nitroanilide ; PS ; polystyrene ; PSMA ; poly(styrene-co-maleic anhydride) ; NPs ; magnetic nanoparticles ; NFs ; nanofibers ; NP-NFs ; magnetic nanoparticle incorporated nanofibers (or magnetic nanofibers) ; EC ; enzyme coating ; EC-TR/NP-NFs (or EC-TR) ; trypsin coatings on magnetic nanofibers ; EC-TR/NPs ; trypsin coatings on magnetic nanoparticles ; CA ; covalent attachment ; CA-TR/NP-NFs (or CA-TR) ; coval
- 刊名：Chemical Engineering Journal
- 出版年：2016
- 出版时间：15 March 2016
- 年：2016
- 卷：288
- 期：Complete
- 页码：770-777
- 全文大小：1383 K
文摘
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Trypsin coating (EC-TR) on magnetically-separable polymer nanofibers was developed.
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Highly active and stable EC-TR was successfully employed for fast protein digestion.
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Ultra-sonication with EC-TR expedited protein digestion process.
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EC-TR showed no performance decrease in its recycled used for protein digestion.