A naked-eye visible and turn-on fluorescence probe for Fe³⁺ and its bioimaging application in living cells

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• 作者：Yaming Liu^aAuthor Vitae ; Jinyu Zhang^bAuthor Vitae ; Jiaxi Ru^aAuthor Vitae ; Xiang Yao^aAuthor Vitae ; Yang Yang^aAuthor Vitae ; Xinghua Li^aAuthor Vitae ; Xiaoliang Tang^aAuthor Vitae ; Guolin Zhang^a ; ^{zhanggl@lzu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Weisheng Liu^a ; ^{liuws@lzu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Naked-eye ; Turn-on ; Fluorescence probe ; Fe3+ ; Bioimaging
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：237
• 期：Complete
• 页码：501-508
• 全文大小：2669 K

文摘

A novel turn-on fluorescence probe based on rhodamine B for Fe³⁺ has been synthesized and characterized by various analytical techniques. Compared with other commonly coexisting metal ions, the probe showed 100-fold fluorescence enhancement for detecting Fe³⁺ with a 1:1 stoichiometry in MeOH/H₂O (1:1, v/v) solution. After adding Fe³⁺, the rapid enhancement of fluorescence intensity provided a rapid method for detection of Fe³⁺. The color change was visible, so it could be used for naked-eye detection. The association constant between the probe and Fe³⁺ was detected to be 0.67 × 10⁴ M⁻¹ and the corresponding detection limit were calculated to be 5.7 × 10⁻⁸ M according to fluorescence titration analysis. The reversible binding of probe L to Fe³⁺ was confirmed by reacting with Na₄P₂O₇, which captured the Fe³⁺ from the L-Fe³⁺complex and turned off the L-Fe³⁺complex. Fluorescence imaging experiments demonstrated that this probe was cell permeable and suitable for monitoring intracellular Fe³⁺ in living cells by confocal microscopy. What’s more, MTT assay indicated that this probe had no significant cytotoxicity to living cells.