Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer
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- 作者：Takayuki Nakagawa ; Hiroyuki Endo ; Misa Yokoyama ; Jiro Abe ; Keiichi Tamai ; Nobuyuki Tanaka ; Ikuro Sato ; Satomi Takahashi ; Takashi Kondo ; Kennichi Satoh
- 关键词：cDNA ; complementary DNA ; EGFR ; epidermal growth factor receptor ; EMT ; epithelial-mesenchymal transition ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; EV cells ; empty vector transfected cells ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; H3K27
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2013
- 出版时间：28 June, 2013
- 年：2013
- 卷：436
- 期：2
- 页码：319-324
- 全文大小：684 K
文摘
HOTAIR is one of long non-coding RNAs and its expression correlates with the prognosis and metastasis in various cancers. We showed that HOTAIR expression has an important role in the development of non-small cell lung cancer (NSCLC). In this study, we examined the expression of HOTAIR in 77 NSCLCs, their corresponding normal lung tissues and 6 brain metastases by quantitative real-time RT-PCR. High expression of HOTAIR (tumor/normal ratio ?2) was detected in 17 patients (22.1 % ) and was frequently found in patients with advanced stage, lymph node metastasis or lymph-vascular invasion and short disease free interval. Furthermore, brain metastases show significantly higher HOTAIR expression compared to primary cancer tissues. HOTAIR-expressing A549 cells showed induced cell migration and anchorage-independent cell growth in vitro. These results indicate the expression of HOTAIR enhanced the aggressive behavior of NSCLC cells.