Large-scale separation of ricinine from a by-product of Ricinus communis L. by pH-zone-refining counter-current chromatography

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• 作者：Jiuming Li ; Dongyu Gu ; Ying Liu ; Fenglan Huang ; Yi Yang
• 关键词：Ricinus communis L. ; By-product ; Active composition ; Ricinine ; pH-zone-refining counter-current chromatography
• 刊名：Industrial Crops and Products
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：49
• 期：Complete
• 页码：160-163
• 全文大小：738 K

文摘

Castor bean meal is the by-product of castor oil production with many active components. One of the major active compounds in castor bean meal is ricinine. In the present study, a pH-zone-refining counter-current chromatography (CCC) method was developed and established to separate ricinine from the by-product of Ricinus communis L. The CCC separation was performed with a two-phase solvent system composed of MtBE-CH₃CN-water (2:2:3, v/v) at a flow rate of 2.0 mL/min, 10 mM triethylamine (TEA) was added to the upper phase as a retainer and 10 mM HCl to the lower phase as an eluter. As a result, 496 mg of ricinine with the purity of 95.1 % was obtained from 2 g crude sample in a single run, and the structure was confirmed by MS, ¹H NMR and ¹³C NMR. Experimental results showed that the present method is time-saving, high sample recovery and very suitable for large-scale isolation of ricinine.