Rho activation is required for transforming growth factor-β-induced epithelial-mesenchymal transition in lens epithelial cells

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• 作者：Hee Jun Cho ; Jiyun Yoo
• 关键词：Transforming growth factor-β ; Epithelial-mesenchymal transition ; Smad ; Rho ; ROCK
• 刊名：Cell Biology International
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：31
• 期：10
• 页码：1225-1230
• 全文大小：2504 K

文摘

Lens epithelial cells undergo epithelial-mesenchymal transition (EMT) after injury as in cataract extraction, leading to fibrosis of the lens capsule. We have recently shown that TGF-β-induced EMT in lens epithelial cells depends on PI3 kinase/Akt signal pathway. In this report, we suggest Smad3 is necessary for TGF-β-induced EMT by showing that the expression of dominant-negative Smad3 blocks the expression of α-smooth muscle actin (α-SMA) and morphological changes. We also show that TGF-β induces a biphasic change in Rho activity, and that Y27632, a selective inhibitor of Rho effector ROCK, inhibits TGF-β-induced EMT in vitro and in vivo. We finally show that Smad3 activation and Rho signal activation is independent each other. All of these findings suggest that Rho/ROCK activation together with Smad3 is necessary for TGF-β-induced EMT in lens epithelial cells.