Rapid detection of novel caprine parainfluenza virus type 3 (CPIV3) using a TaqMan-based RT-qPCR
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- 作者：Jizong Li ; Wenliang Li ; Li Mao ; Fei Hao ; Leilei Yang ; Wenwen Zhang ; Jieyuan Jiang ; 1776556843@qq.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：TaqMan RT-qPCR ; Caprine parainfluenza virus type 3 ; Sample detection
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：126-131
- 全文大小：859 K
文摘
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A TaqMan-based real-time RT-PCR was developed for CPIV3 detection.
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Primers and probe were designed based on the alignment of the M gene.
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The method showed high sensitivity, detecting ten copies of CPIV3-RNA.
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The method allows the measurement of CPIV3 virus load in different tissues.