Effects of cyclopenta[c]phenanthrene and its derivatives on zona radiata protein, ERα, and CYP1A mRNA expression in liver of rainbow trout (Oncorhynchus mykiss Walbaum)

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• 作者：Maciej Woź ; ny ; Pawe&#x142 ; Brzuzan ; Micha&#x142 ; K. &#x141 ; uczy&#x144 ; ski ; Maciej Gó ; ra ; Joanna Bidzi&#x144 ; ska ; Pawe&#x142 ; Jurkiewicz
• 关键词：Cyclopenta[c]phenanthrenes ; CYP1A mRNA ; Estrogen receptor ; //www.sciencedirect.com/scidirimg/entities/204e.gif"" alt=""greek small letter alpha"" title=""greek small letter alpha"" border=""0""> mRNA ; Rainbow trout ; Zona radiata prote
• 刊名：Chemico-Biological Interactions
• 出版年：2008
• 出版时间：10 July 2008
• 年：2008
• 卷：174
• 期：1
• 页码：60-68
• 全文大小：236 K

文摘

Despite cyclopenta-fused polycyclic aromatic hydrocarbons (CP-PAHs) have been detected in the environment, the ability of CP-PAH to induce cellular and tissue responses remains poorly characterized. In this study, xenoestrogen-associated responses (mRNA levels of estrogen receptor , ER, and zona radiata protein, Zrp) and xenobiotic effects (CYP1A mRNA) have been investigated in liver of juvenile rainbow trout after short-term treatment (8 and 24 h) with following compounds administered singly: cyclopenta[c]phenanthrene (CP[c]Ph); its derivatives, 5A-CP[c]Ph; 5A6M-CP[c]Ph; 5A9M-CP[c]Ph; B[c]Ph, a structurally similar polycyclic aromatic hydrocarbon; B[a]P, a model CYP1A inducer; and zearalenone (ZEA), naturally occurring ligand for ER. The CYP1A mRNA expression after 24 h of exposure with CP[c]Ph or its derivatives, except 5A9M-CP[c]Ph, was 3–9-fold higher compared to controls (P < 0.05), but it was less than that caused by B[a]P (65-fold up regulation; P < 0.01). Moreover, neither of the CP-PAH compounds modulated liver ER or Zrp mRNA levels as compared to effects associated with ZEA. Interestingly, a treatment with this ER-ligand, caused moderate but significant increase of CYP1A mRNA expression (about 2.5-fold; P < 0.05). The finding that ZEA is capable of acting as either estrogenic and xenobiotic compound, should be further explored in a more detailed and differently designed experiment.