A Strategy to Combine Sample Multiplexing with Targeted Proteomics Assays for High-Throughput Protein Signature Characterization

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• 作者：Brian K. Erickson¹ ; ⁴ ; Christopher M. Rose¹ ; ⁴ ; Craig R. Braun¹ ; Alison R. Erickson¹ ; ² ; Jeffrey Knott³ ; Graeme C. McAlister¹ ; Martin Wü ; hr¹ ; Joao A. Paulo¹ ; Robert A. Everley¹ ; ² ; ⁵ ; ^{Robert_Everley@hms.harvard.edu} ; Steven P. Gygi¹ ; ^{Steven_Gygi@hms.harvard.edu}
• 关键词：proteomics ; targeted proteomics ; multiplexing ; isobaric tag ; high-throughput ; cancer ; NCI-60
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：19 January 2017
• 年：2017
• 卷：65
• 期：2
• 页码：361-370
• 全文大小：3556 K
• 卷排序：65

文摘

Combined peptide and sample multiplexing increases throughput for targeted analyses Unfractionated targeted 2D multiplexing reproduces global proteome quantitation Monitored 69 proteins across 180 cancer cell lines in 48 hr (16 min/sample) NCI-60 profiling revealed correlation of BAZ1B expression and doxorubicin response