Acute protective effect of nimodipine and dimethyl sulfoxide against hypoxic and ischemic damage in brain slices

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• 作者：Greiner ; Christoph ; Schmidinger ; Andrea ; Hü ; lsmann ; Swen ; Moskopp ; Dag ; Wö ; lfer ; Johannes ; et. al.
• 关键词：Disorders of the nervous system ; Ischemia ; Ischemia ; Brain slice ; Neuroprotection ; Nimodipine ; Dimethyl sulfoxide
• 刊名：Brain Research
• 出版年：2000
• 出版时间：December 29, 2000
• 年：2000
• 卷：887
• 期：2
• 页码：316-322
• 全文大小：160 K

文摘

Nimodipine and dimethyl sulfoxide (DMSO) were tested (alone and in combination) regarding their ability to increase hypoxic tolerance of brain slices under ‘hypoxic’ (deprivation of oxygen) or ‘ischemic’ (hypoxia+withdrawal of glucose) conditions. Direct current (DC) and evoked potentials were recorded in the CA1 region of hippocampal slices of adult guinea pigs. After induction of hypoxia or ischemia, the latency of anoxic terminal negativity (ATN) of the DC potential was determined during superfusion with artificial cerebrospinal fluid alone (aCSF), and during superfusion with aCSF containing DMSO [0.1 % (14.1 mmol/l) and 0.4 % (56.3 mmol/l)] with the addition of nimodipine (40 μmol/l). Latencies of ATN with first hypoxia were 6.7±3.7 min in the control group, 9.3±4.2 min in the 0.4 % DMSO group and 12.3±5.5 min (P