Converting a Protease Assay to a Caliper® Format LabChip System

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• 作者：Michael Johnson ; Chuck Li ; Brian Rasnow ; Peter Gr ; sard ; Hong Xing ; Angelica Fields
• 关键词：Author Keywords ; MINIATURIZATION ; LAB CHIP ; COMPOUND SCREENING
• 刊名：Journal of the Association for Laboratory Automation
• 出版年：2002
• 出版时间：1 August 2002
• 年：2002
• 卷：7
• 期：4
• 页码：62-68
• 全文大小：2303 K

文摘

The push for higher throughput screening coupled with the desire to use smaller volumes of material has sparked the development of new technologies. Caliper Technologies, Corp. (Mountain View, CA) has designed a microfluidics chip with unique properties yet to be fully exploited. The translation from a traditional plate-based assay to a microfluidic chip format has provided insights into assay development, screening data requirements, and the technology itself. Running a screen with this new technology presented challenges in throughput, signal acquisition from slow-conversion enzymes, the provision for a negative control, the translation of a time series into a single data point per compound, reagent adhesion in the channels, and fluid property mismatches. Overcoming these obstacles has resulted in a simple, robust system with significant savings in reagent use. Measures to improve throughput and generalize the system will be discussed.