A novel method for a fast diagnosis of septic arthritis using mid infrared and deported spectroscopy

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• 作者：Jean-David Albert^a ; ^b ; ^{jean-david.albert@chu-rennes.fr" class="auth_mail" title="E-mail the corresponding author} ; ^{albert.jean-david@wanadoo.fr" class="auth_mail" title="E-mail the corresponding author} ; Val&eacute ; rie Monbet^c ; ^d ; ^e ; Anne Jolivet-Gougeon^f ; Nadia Fatih^g ; Maë ; na Le Corvec^g ; ^h ; Malik Seck^c ; Fr&eacute ; d&eacute ; ric Charpentier^g ; Guillaume Coiffier^a ; ^b ; Catherine Boussard-Pledelⁱ ; Bruno Bureauⁱ ; Pascal Guggenbuhl^a ; ^b ; ^c ; Olivier Lor&eacute ; al^b ; ^c
• 关键词：Deported mid infrared spectroscopy ; Synovial fluid ; Arthritis ; Infectious ; Diagnosis
• 刊名：Joint Bone Spine
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：83
• 期：3
• 页码：318-323
• 全文大小：826 K

文摘

To assess the ability of mid infrared deported spectroscopy to discriminate synovial fluids samples of septic arthritis patients from other causes of joint effusion.

absSec_2">Methods

ar0010">Synovial fluids obtained from patients with clinically suspected arthritis were collected, analysed and classified according to standard diagnostic procedures as septic or non-septic. A spectroscopic analysis on synovial fluid samples was then performed using a coiled optical fiber made with chalcogenide glass. After a factorial analysis of the normalized spectra and the computation of a Fisher test used to select the most relevant components, a logistic regression model was fitted, allowing to attribute a score between 0 – non-septic –, and 1 – septic.

absSec_3">Results

ar0015">In a first phase, we analysed the synovial fluids from 122 different synovial fluids including 6 septic arthritis among arthritis of various origins. Septic synovial fluids were identified with a sensitivity of 95.8% and a specificity of 93.9% and an AUROC of 0.977. The analysis of an independent set of samples (n = 42, including two septic arthritis) gave similar values.

absSec_4">Conclusions

ar0020">Our data strongly supports the interest of mid infrared deported spectroscopy, which could be used potentially at point of care, for a rapid and easy diagnosis of septic arthritis. Now, the precision of the diagnosis must be evaluated through a multicentric study on a larger panel of patients.