Expression in Arabidopsis

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• 作者：Li-mei YANG ; Per Mercke ; Joop JA van Loon ; Zhi-yuan FANG ; Marcel Dicke ; Maarten A Jongsma
• 关键词：Aradidopsis ; linalool synthase ; potato proteins inhibitor ; inducible promoter ; methyl jasmonate ; Plutella xylostella ; induction ; expression
• 刊名：Agricultural Sciences in China
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：7
• 期：5
• 页码：521-534
• 全文大小：1197 K

文摘

To investigate the role of inducible linalool in Arabidopsis-insect interactions, the FaNES1 linalool synthase (LIS) cDNA from strawberry with plastid targeting and a synthetic intron (LIS') was placed under the control of the wound inducible proteinase inhibitor 2 (PI2) promoter from potato. The construct pBin-P_PI2-LIS' was transformed to Arabidopsis thaliana ecotype Columbia 0. Kanamycin resistant T₀ seedlings were confirmed for the presence and transcription of the LIS' gene by PCR analysis on genomic DNA and by RT-PCR analysis on RNA. Genomic and RT-PCR products were sequenced to confirm correct splicing of the synthetic intron. The expression of active linalool synthase by the P_PI2-LIS' gene construct in the transgenic lines was assessed by measuring linalool emission using solid phase micro-extraction (SPME) GC-MS measurements after induction with methyl jasmonate. Among 30 tested independent T₂ transgenic lines, 10 exhibited linalool production. Linalool expression could be induced by methyl jasmonate treatment, but not by diamondback moth larvae.