HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing

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• 作者：Jorge Fraga ; Ana M. Montalvo ; llse Maes ; Jean-Claude Dujardin ; Gert Van der Auwera
• 关键词：Leishmania ; Typing ; hsp70 ; PCR-RFLP
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：77
• 期：3
• 页码：245-247
• 全文大小：479 K

文摘

Restriction fragment length polymorphisms of the heat-shock protein 70 gene have been used for discriminating Leishmania species. Here, we validated HindII as a much cheaper alternative to EcoRII and SduI for discriminating Leishmania (Viannia) braziliensis from Leishmania (Viannia) naiffi and an atypical Leishmania (V.) braziliensis group, which was previously not possible.