Evaluation of basic mitochondrial functions using rat tissue homogenates

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• 作者：Alena Pecinová ; ^a ; ^{alena.pecinova@biomed.cas.cz"" rel=""nofollow} ; Zden&#x11b ; k Drahota^a ; ^{drahota@biomed.cas.cz"" rel=""nofollow} ; Hana Nů ; sková ; ^a ; ^{nuskova@biomed.cas.cz"" rel=""nofollow} ; Petr Pecina^a ; ^{petr.pecina@biomed.cas.cz"" rel=""nofollow} ; Josef Houš ; t&#x11b ; k ; ^a ; ^{houstek@biomed.cas.cz"" rel=""nofollow}
• 关键词：Isolated mitochondria ; Tissue homogenates ; oxidative phosphorylation ; Respiratory control ; Mitochondrial membrane potential
• 刊名：Mitochondrion
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：11
• 期：5
• 页码：722-728
• 全文大小：766 K

文摘

The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50–100 mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria.