Erratum to “Factors associated with longitudinal plasma selenium decline in the elderly: The EVA Study” [J Nutr Biochem 18 (2007) 482-487]
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- 作者：Josiane Arnaud ; N. Tasmine Akbaraly ; Isabelle Hininger ; Anne-Marie Roussel ; Claudine Berr
- 关键词：CCS ; SOD1 ; Copper status ; Ceruloplasmin ; Real-time PCR
- 刊名：Journal of Nutritional Biochemistry
- 出版年：2008
- 出版时间：April 2008
- 年：2008
- 卷：19
- 期：4
- 页码：275
- 全文大小：87 K
文摘
The limits of copper homeostatic regulation in humans are not known, making it difficult to define the milder effects of early copper excess. Furthermore, a robust assay to facilitate the detection of early stages of copper excess is needed. To address these issues, we assessed changes in relative mRNA abundance of methallothionein 2A (MT2A), prion (PrP), amyloid precursor-like protein 2 (APLP2), Cu/Zn superoxide dismutase (SOD1) and its copper chaperone (CCS) in peripheral mononuclear cells (PMNCs) from healthy adults representing the 5 % highest and lowest extremes in the distribution curve of serum ceruloplasmin (Cp) concentrations of 800 individuals. The intracellular Cu content was also determined. PMNCs were isolated from individuals before and after exposure to a single daily dose of 10 mg Cu (as CuSO4) for 2 months. Results showed that although there were fluctuations in serum Cp values of the samples assessed before copper exposure, no significant differences were observed in cell copper content or in the relative abundance of MT2A, PrP and APLP2 transcripts in PMNCs. Also, these values were not modified after copper supplementation. However, CCS and SOD1 mRNA levels were reduced in PMNCs after copper supplementation in the individuals with the high Cp values, suggesting that they should be further explored as biomarkers of moderate copper overload in humans.