Influence of size, surface coating and fine chemical composition on the in vitro reactivity and in vivo biodistribution of lipid nanocapsules versus lipid nanoemulsions in cancer models

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• 作者：Samuli Hirsjä ; rvi ; PhD^c ; ¹ ; Sandrine Dufort ; PhD^a ; ^b ; ¹ ; Julien Gravier ; PhD^d ; ¹ ; Isabelle Texier ; PhD^d ; Qiao Yan ; PhD^e ; Jé ; rome Bibette ; PhD^e ; Lucie Sancey ; PhD^a ; ^b ; Vé ; ronique Josserand ; PhD^a ; ^b ; Catherine Passirani ; PhD^c ; Jean-Pierre Benoit ; PhD^c ; Jean-Luc Coll ; PhD^a ; ^b ; ^{Jean-Luc.Coll@ujf-grenoble.fr}
• 关键词：Biocompatible materials ; Biodistribution ; Lipid nanocarriers ; Nanoparticles ; Near-infrared optical imaging
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：9
• 期：3
• 页码：375-387
• 全文大小：1942 K

文摘

Lipid nanocapsules (LNCs) and lipid nanoemulsions (LNEs) are biomimetic synthetic nanocarriers. Their in vitro and in vivo performance was evaluated as a function of their size (25, 50 and 100 nm) and the surface PEG chain length. Analysis methods included complement activation test, particle uptake in macrophage and HEK293(¦Â3) cells and biodistribution studies with tumor-grafted mice by fluorescence imaging. A particular attention was paid to keep the concentration of each nanocarrier and to the amount of fluorescent dye in comparable conditions between the in vitro and in vivo studies. Under these conditions, no significant differences were found among the three tested particle sizes and the two nanocarrier types. Longer PEG chains on the LNE surface provided better stealth properties, whereas PEG modification on the LNC formulations inhibited the production of stable nanocarriers. Passive accumulation of LNCs and LNEs in different tumor types depended on the degree of tumor vascularization.

From the Clinical Editor

This study of lipid nanocapsules and lipid nanoemulsions compares their vitro and in vivo performance as a function of size and surface PEG chain length, demonstrating no significant difference among the tested particle sizes. Longer PEG chains on the LNE surface provided better stealth properties, whereas PEG modification on the LNC formulations inhibited the production of stable nanocarriers.