The effects of prostaglandin A₂ on cell growth, cell cycle status and apoptosis induction in HeLa and MCF-7 cells

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• 作者：Joubert ; Annie M. ; Panzer ; Annie ; Bianchi ; Pepita C. ; Lottering ; Mona-Liza
• 关键词：Prostaglandin A₂ ; Cell numbers ; Apoptosis
• 刊名：Cancer Letters
• 出版年：2003
• 出版时间：March 10, 2003
• 年：2003
• 卷：191
• 期：2
• 页码：203-209
• 全文大小：289 K

文摘

The effects of 20 μg/ml exogenous prostaglandin A₂ (PGA₂) were evaluated on cell numbers in HeLa (human epithelial cervix carcinoma) and MCF-7 (human breast carcinoma) cells. In HeLa cells, PGA₂ reduced cell numbers significantly to 75 % after 24 h (P<0.05) and exposure of 48 h decreased cell numbers to 61 % (P<0.05) of the control. In MCF-7 cells, PGA₂ significantly reduced cell numbers to 48 % after 24 h and to 20 % after 48 h, compared to vehicle-treated control cells (P<0.05). The anti-mitogenic effects were confirmed by morphological studies conducted after 48 h of exposure to PGA₂, when optimal effects were observed. HeLa and MCF-7 cells exposed to PGA₂, showed chromatin aggregation, cell membrane blebbing and uneven distribution of chromosomes. Cell cycle progression analysis of HeLa and MCF-7 cells, showed an increase in DNA content preceding the G₀/G₁ peak after 48 h of exposure, which is indicative of apoptotic body formation.