Inhibition of UVB-induced wrinkle formation and MMP-9 expression by mangiferin isolated from Anemarrhena asphodeloides

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• 作者：Hui-Seong Kim ; Jae Hyoung Song ; Ui Joung Youn ; Jin Won Hyun ; Woo Seok Jeong ; Mi Young Lee ; Hwa Jung Choi ; Hyeong-Kyu Lee ; Sungwook Chae
• 关键词：Ultraviolet B ; MMP-9 (Matrix metalloproteinase) ; Mangiferin ; Skin aging
• 刊名：European Journal of Pharmacology
• 出版年：2012
• 出版时间：15 August, 2012
• 年：2012
• 卷：689
• 期：1-3
• 页码：38-44
• 全文大小：661 K

文摘

Chronic exposure of human skin to solar ultraviolet (UV) radiation causes photoaging. Naturally occurring phytochemicals are known to have anti-photoaging effects. The present study examined the effect of mangiferin isolated from Anemarrhena asphodeloides on wrinkle formation, skin thickness, and changes in collagen fibers in hairless mice. The in vitro effects and possible mechanism of mangiferin on UVB irradiation were determined in human keratinocyte (HEKa) cells. In vitro results showed that mangiferin reduced UVB-induced matrix metalloproteinase (MMP)-9 protein expression and enzyme activity and subsequent attenuation of UVB-induced phosphorylation of mitogen-activated protein kinase kinase1 (MEK) and extracellular signal-regulated kinase (ERK). In the in vivo studies, mangiferin inhibited UVB-induced mean length and mean depth of skin wrinkle based on skin replica, epidermal thickening, and damage to collagen fiber. Taken together, these results indicate that mangiferin exerts anti-photoaging activity in UVB-irradiated hairless mice by regulating MMP-9 expression through inhibition of MEK and ERK.