The Bmi-1 oncoprotein is overexpressed in human colorectal cancer and correlates with the reduced p16INK4a/p14ARF proteins
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- 作者：Kim ; Joo Heon ; Yoon ; Sun Young ; Kim ; Chang-Nam ; Joo ; Joung Hyuck ; Moon ; Sang Kyoung ; Choe ; In Seong ; et. al.
- 关键词：Bmi-1 ; Up-regulation ; Colorectal carcinoma ; p16INK4a ; p14ARF
- 刊名：Cancer Letters
- 出版年：2004
- 出版时间：January, 2004
- 年：2004
- 卷：203
- 期：2
- 页码：217-224
- 全文大小：341 K
文摘
To clarify the roles of Bmi-1 in colorectal carcinoma, we examined the expression of Bmi-1 in 41 samples out of 46 colorectal carcinomas by reverse transcription-PCR, whereas all 46 were analyzed by immunostaining. In addition, we analyzed the expression patterns of Bmi-1 in association with p16INK4a and p14ARF (in mouse p19ARF) in a series of colorectal carcinomas. The level of Bmi-1 mRNA in the carcinoma tissues was significantly higher than those of the adjacent non-neoplastic colonic mucosal tissues. Immunohistochemistry for Bmi-1 showed moderate or strong expression levels in 65 % (30/46) of colorectal carcinomas. Colorectal carcinomas with moderate or strong Bmi-1 expression were more likely to have low levels of the INK4 locus proteins (p16INK4a/p14ARF) (P<0.07). These results suggested that modulation of Bmi-1 protein might be involved in human colorectal carcinogenesis by repressing the INK4a/ARF proteins.