RAP80 interacts with the SUMO-conjugating enzyme UBC9 and is a novel target for sumoylation
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- 作者：Jun Yan ; Xiao-Ping Yang ; Yong-Sik Kim ; Joung Hyuck Joo ; Anton M. Jetten
- 关键词：RAP80 ; UBC9 ; SUMO-1 ; Sumoylation
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 出版时间：12 October 2007
- 年：2007
- 卷：362
- 期：1
- 页码：132-138
- 全文大小：730 K
文摘
RAP80, a nuclear protein with two functional ubiquitin-interaction motifs (UIMs) at its N-terminus, plays a critical role in the regulation of estrogen receptor alpha and DNA damage response signaling. A yeast two-hybrid screen identified the SUMO-conjugating enzyme UBC9 as a protein interacting with RAP80. The interaction of RAP80 with UBC9 was confirmed by co-immunoprecipitation and GST pull-down analyses. The region between aa 122–204 was critical for the interaction of RAP80 with UBC9. In addition, we demonstrate that RAP80 is a target for SUMO-1 modification in intact cells. Expression of UBC9 enhanced RAP80 mono-sumoylation and also induced multi-sumoylation of RAP80. In addition to SUMO-1, RAP80 was efficiently conjugated to SUMO-3 but was only a weak substrate for SUMO-2 conjugation. These findings suggest that sumoylation plays a role in the regulation of RAP80 functions.