Overexpression of Bmi-1 oncoprotein correlates with axillary lymph node metastases in invasive ductal breast cancer
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- 作者：Joo Heon Kim ; Sun Young Yoon ; Seong-Hoo Jeong ; Soo Young Kim ; Sang Kyoung Moon ; Joung Hyuck Joo ; Younghee Lee ; In Seong Choe ; Jae Wha Kim
- 关键词：Bmi-1 ; Lymph node metastasis ; Breast carcinoma
- 刊名：The Breast
- 出版年：2004
- 出版时间：October 2004
- 年：2004
- 卷：13
- 期：5
- 页码：383-388
- 全文大小：386 K
文摘
The modulation of Bmi-1 is observed in several tumor tissues, and its heightened protein level is suspected to be involved in tumorigenesis by acting as a transcriptional repressor in the INK4a/ARF locus. To elucidate the modulation of Bmi-1 in invasive ductal breast cancers, we examined its transcript and protein levels. The bmi-1 mRNA level by reverse transcription-polymerase chain reaction (RT-PCR) showed that it was significantly up-regulated in 28 specimens out of 33 breast carcinoma tissues compared with those of non-neoplastic tissues just adjusted to tested specimens. Immunohistochemical staining for Bmi-1 also showed that 44 specimens out of 71 breast carcinoma tissues (62 % ) had strong positive signals with a more intense staining pattern in the invading fronts than in the central portions of primary invasive breast cancers. Univariate and multivariate analyses showed that a high level of Bmi-1 expression was significantly correlated with axillary lymph node metastases and positive estrogen receptor status. These findings suggested that Bmi-1 might be involved in the tumor progression and metastasis of invasive ductal breast cancer.