The protective effect of grape seed procyanidin extract against cadmium-induced renal oxidative damage in mice

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• 作者：Qing Chen ; Rong Zhang ; Wei-min Li ; Yu-jie Niu ; Hui-cai Guo ; Xue-hui Liu ; Yu-chun Hou ; Li-juan Zhao
• 关键词：Cadmium ; Renal damage ; Grape seed procyanidin extract ; Oxidative stress
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：November, 2013
• 年：2013
• 卷：36
• 期：3
• 页码：759-768
• 全文大小：2105 K

文摘

As an important environmental pollutant, cadmium (Cd) can lead to serious renal damage. Grape seed procyanidins extract (GSPE), a biological active component of grape seed, has been shown to possess antioxidative effects. Here, we assessed the protective effect of GSPE on Cd-induced renal damage using animal experiment. After 30 days, the oxidative damage of kidney was evaluated through measurement of superoxide dismutase (SOD), glutathione peroxidation (GSH-Px) and malondialdehyde (MDA). Since, oxidative stress could lead to apoptosis, the renal apoptosis was measured using flow cytometer. Moreover, the expression of apoptosis-related protein Bax and Bcl-2 was analyzed by immunohistochemistry and Western blot. The results showed that Cd led to the decrease of SOD and GSH-Px activities, and the increase of MDA level, induced renal apoptosis. However, the coadministration of GSPE attenuated Cd-induced lipid peroxidation, and antagonized renal apoptosis, probably associated with the expression of Bax and Bcl-2. These data suggested that GSPE has protective effect against renal oxidative damage induced by Cd, which provide a potential natural chemopreventive agent against Cd-poisoning.