Enantioselective and sensitive determination of carvedilol in human plasma using chiral stationary-phase column and reverse-phase liquid chromatography with tandem mass spectrometry
详细信息 查看全文
- 作者：Juanjuan Jiang jiangjuanjuan_80@163.com" class="auth_mail ; Lei Tian tianlei0807@hotmail.com" class="auth_mail ; Yiling Huang hylhyl@foxmail.com" class="auth_mail ; Yan Yan YanY926@hotmail.com" class="auth_mail ; Yishi Li ; Liyishi@public3.bta.net.cn" class="auth_mail
- 关键词：Carvedilol ; Enantiomer ; Chiral column ; LC&ndash ; MS/MS ; Human plasma ; Method validation
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：1 June 2014
- 年：2014
- 卷：960
- 期：Complete
- 页码：92-97
- 全文大小：896 K
文摘
- •
Enantioselective separation of carvedilol by RP–HPLC–MS/MS and chiral stationary-phase column has been developed.
- •
Excellent resolution of carvedilol enantiomers is achived.
- •
The chiral stationary-phase columns and the impact factors for seperation and MS response are detailed.
- •
Compared to the reported methods, the present method is simpler, more rapid and sensitive.
- •
This method was successfully applied to quantify plasma samples from a phase I clinical trial of carvedilol.