A rapid and sensitive LC-MS/MS-ESI method for the determination of tolvaptan and its two main metabolites in human plasma
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- 作者：Juanjuan Jiang jiangjuanjuan_80@163.com" class="auth_mail" title="E-mail the corresponding author ; Lei Tian tianlei0807@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Yiling Huang hylhyl@foxmail.com" class="auth_mail" title="E-mail the corresponding author ; Yan Yan YanY926@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Yishi Li ; Liyishi@public3.bta.net.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Tolvaptan ; Metabolite ; LC&ndash ; MS/MS ; Human plasma ; Method validation
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 August 2016
- 年：2016
- 卷：1027
- 期：Complete
- 页码：158-164
- 全文大小：1179 K
文摘
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This validated method for tolvaptan and its two main metabolites for use in a human pharmacokinetic study has not previously been reported.
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An RP-HPLC–MS/MS method for tolvaptan and its two main metabolites in vivo was developed and fully validated.
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This method was successfully used to quantify tolvaptan and its two main metabolites in plasma samples from a phase I clinical trial of tolvaptan.
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The specificity and sensitivity, the chromatography conditions, and the sample pre-treatment procedure are reported.
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Compared to previously published methods, this RP-HPLC–MS/MS method is easier to perform, more rapid and has higher sensitivity.