235: Effects of dietary fructo-oligosaccharides on gender-based differences in gut mucosal cytokines

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• 作者：Padmaja Shastri ; Justin McCarville ; Stephen P.J. Brooks ; Martin Kalmokoff ; Julia M. Green-Johnson
• 刊名：Cytokine
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：63
• 期：3
• 页码：298
• 全文大小：40 K

文摘

Gut microbiota-mediated effects on the immune system can influence cytokine production in gut-associated lymphoid tissues (GALT). The extent to which gender-associated differences in gut microbiota composition influence mucosal cytokine patterns is as yet undetermined, as is the impact such differences may have on the ability of fermentable material to modulate immune activity through effects on the gut microbiota. To explore these questions, we analyzed the effects of a fructo-oligosaccharide (FOS)-supplemented Purina chow diet on mucosal cytokine profiles, focusing on IL-6 (pro-inflammatory), IL-4 (TH₂) and IL-10 (regulatory T_reg). To compare gender-associated effects of FOS at the gut microbial and intestinal epithelial level, we measured fecal short chain fatty acid profiles (by gas chromatography) and mucin gene MUC2 expression (determined by quantitative real-time PCR). No major FOS-induced changes in cytokine profiles (vs. control) were observed in the ileum of male or female rats, although gender-specific differences in cytokine levels were observed. Cecal IL-6 (7732.49 ¡À 863 pg/g) was higher in FOS-fed male rats compared to FOS-fed female rats (5057.9 ¡À 536.5 pg/g). Cecal IL-10 was significantly lower in male rats fed the control diet (5301.2 ¡À 241.6 pg/g) relative to female rats fed the same diet (8164 ¡À 843.6 pg/g). However, cecal IL-10 was elevated in FOS-fed male rats (6484.3 ¡À 1269.7 pg/g) to levels similar to those of FOS-fed female rats (6662.4 ¡À 335.6 pg/g), indicating a potential differential effect of dietary FOS on this regulatory cytokine in males and females. Fecal butyric acid concentrations in FOS-fed male rats (35.1 ¡À 7.1 mmol/g) were significantly higher than those in FOS-fed female rats (13.6 ¡À 2.6 mmol/g), suggesting a gender-associated difference in the effects of FOS at the gut microbial level. Preliminary results indicate higher cecal MUC2 expression in male rats relative to female rats. These findings illustrate that fermentation-associated luminal changes may affect GALT immune activity in a gender-specific fashion.