A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging

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• 作者：Jae-Hwan You ; Gareth Howell ; Asit K. Pattnaik ; Fern ; o A. Osorio ; Julian A. Hiscox
• 关键词：Nuclear export ; Nuclear import ; PRRSV ; Nucleocapsid protein ; Nucleolus ; Nucleus ; Cytoplasm ; Confocal ; FLIP ; FRAP ; Arterivirus ; Virus ; Nucleolar
• 刊名：Virology
• 出版年：2008
• 出版时间：15 August 2008
• 年：2008
• 卷：378
• 期：1
• 页码：34-47
• 全文大小：4730 K

文摘

Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, in common with many other positive strand RNA viruses, encodes a nucleocapsid (N) protein which can localise not only to the cytoplasm but also to the nucleolus in virus-infected cells and cells over-expressing N protein. The dynamic trafficking of positive strand RNA virus nucleocapsid proteins and PRRSV N protein in particular between the cytoplasm and nucleolus is unknown. In this study live imaging of permissive and non-permissive cell lines, in conjunction with photo-bleaching (FRAP and FLIP), was used to investigate the trafficking of fluorescent labeled (EGFP) PRRSV-N protein. The data indicated that EGFP-PRRSV-N protein was not permanently sequestered to the nucleolus and had equivalent mobility to cellular nucleolar proteins. Further the nuclear import of N protein appeared to occur faster than nuclear export, which may account for the observed relative distribution of N protein between the cytoplasm and the nucleolus.