Identification of a novel starch synthase III from the picoalgae Ostreococcus tauri
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- 作者：Julieta Barchiesi<sup>asup> ; Nicolá ; s Hedin<sup>asup> ; Alberto A. Iglesias<sup>csup> ; Diego F. Gomez-Casati<sup>asup> ; Miguel A. Ballicora<sup>bsup> ; Marí ; a V. Busi<sup>asup><sup> ; sup><sup>busi@cefobi-conicet.gov.arsup>
- 关键词：O. tauri ; Starch synthase ; Protein activity
- 刊名：Biochimie
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：133
- 期：Complete
- 页码：37-44
- 全文大小：2374 K
- 卷排序：133
文摘
We characterized a starch synthase III (SSIII) isoform from O. tauri which lacks starch binding domains (SBDs). Recombinant OsttaSSIII-C exhibited preference to branched glycans such as glycogen and amylopectin, rather than amylose. Results suggest a strong similarity between the active site of the O. tauri SSIII-C and other bacterial glycogen synthases. Mutations in residues R270, K275 and E352 affect OsttaSSIII-C catalytic activity.