Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity
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- 作者：Federico Sisti<sup>asup><sup> ; sup><sup>federico@biol.unlp.edu.arsup> ; Julieta Ferná ; ndez<sup>asup> ; André ; s Cordero<sup>asup> ; Adriana Casabuono<sup>bsup> ; Alicia Couto<sup>bsup> ; Daniela Hozbor<sup>asup>
- 关键词：Lipopolysaccharide ; Bordetella ; Glycosyltransferase
- 刊名：Bioorganic & Medicinal Chemistry Letters
- 出版年：2017
- 出版时间：1 February 2017
- 年：2017
- 卷：27
- 期：3
- 页码：432-436
- 全文大小：712 K
- 卷排序：27
文摘
Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthesis are still unknown. In this context we searched in B. bronchiseptica genome for putative glycosyltransferases. We found possible genes codifying for enzymes involved in sugar substitution of the LPS structure. We decided to analyse BB3394 to BB3400 genes, closed to a previously described LPS biosynthetic locus in B. pertussis. Particularly, conservation of BB3394 in sequenced B. bronchiseptica genomes suggests the importance of this gene for bacteria normal physiology. Deletion of BB3394 abolished resistance to naive serum as described for other LPS mutants. When purified LPS was analyzed, differences in the LPS core structure were found. Particularly, a GalNA branched sugar substitution in the core was absent in the LPS obtained from BB3394 deletion mutant. Absence of GalNA in core LPS alters immune response in vivo but is able to induce protective response against B. bronchiseptica infection.