Identification and molecular characterization of dorsal and dorsal-like genes in the cyclopoid copepod Paracyclopina nana

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• 作者：Chang-Bum Jeong^a ; ^b ; ¹ ; Min Chul Lee^a ; ¹ ; Kyun-Woo Lee^b ; Jung Soo Seo^c ; Heum Gi Park^d ; ^{hgpark@gwnu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Jae-Sung Rhee^e ; ^{jsrhee@incheon.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Jae-Seong Lee^a ; ^{jslee2@skku.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Copepod ; Paracyclopina nana ; Dorsal ; Lipopolysaccharide ; Culture conditions
• 刊名：Marine Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：part_P3
• 页码：319-327
• 全文大小：795 K

文摘

To date, knowledge of the immune system in aquatic invertebrates has been reported in only a few model organisms, even though all metazoans have an innate immune system. In particular, information on the copepod's immunity and the potential role of key genes in the innate immune systems is still unclear. In this study, we identified dorsal and dorsal-like genes in the cyclopoid copepod Paracyclopina nana. In silico analyses for identifying conserved domains and phylogenetic relationships supported their gene annotations. The transcriptional levels of both genes were slightly increased from the nauplius to copepodid stages, suggesting that these genes are putatively involved in copepodid development of P. nana. To examine the involvement of both genes in the innate immune response and under stressful conditions, the copepods were exposed to lipopolysaccharide (LPS), different culture densities, salinities, and temperatures. LPS significantly upregulated mRNA expressions of dorsal and dorsal-like genes, suggesting that both genes are transcriptionally sensitive in response to immune modulators. Exposure to unfavorable culture conditions also increased mRNA levels of dorsal and dorsal-like genes. These findings suggest that transcriptional regulation of the dorsal and dorsal-like genes would be associated with environmental changes in P. nana.