Microbial transformation of ginsenoside-Rg₁ by Absidia coerulea and the reversal activity of the metabolites towards multi-drug resistant tumor cells

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• 作者：Xin  ; Liu^a ; Lirui  ; Qiao^a ; Dan  ; Xie^a ; Yi  ; Zhang^a ; Jianhua  ; Zou^a ; Xiaoguang  ; Chen^a ; Jungui  ; Dai ;   ; ^a ;   ; ^{jgdai@imm.ac.cn}
• 关键词：Microbial transformation ; Ginsenoside-Rg₁ ; Absidia coerulea AS 3.2462 ; Dehydrogenation ; Hydroxylation ; Tumor MDR reversal activity
• 刊名：Fitoterapia
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：82
• 期：8
• 页码：1313-1317
• 全文大小：381 K

文摘

Biotransformation of ginsenoside-Rg₁ (1) by the fungus Absidia coerulea AS 3.2462 yielded five metabolites (2?6). On the basis of spectroscopic data analyses, the metabolites were identified as ginsenoside-F₁ (2), 6¦Á,12¦Â-dihydroxydammar-3-one-20(S)-O-¦Â-D-glucopyranoside (3), 3-oxo-20(S)-protopanaxatriol (4), 3-oxo-7¦Â-hydroxy-20(S)-protopanaxatriol (5), and 3-oxo-7¦Â,15¦Á-dihydroxy-20(S)-protopanaxatriol (6), respectively. Among them, 5 and 6 are new compounds. These results indicated that Absidia coerulea AS 3.2462 could catalyze the specific C-3 dehydrogenation of derivatives of ginsenoside-Rg₁, as well as hydroxylation at the 7¦Â and 15¦Á positions. Metabolites 2, 4 and 5 exhibited moderate reversal activity towards A549/taxol MDR tumor cells in vitro.