Vanadium accelerates polymerase chain reaction and expands the applicability of?forensic DNA testing

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• 作者：Junko Kaminiwa ; MS (Nursing Scientist) ; Katsuya Honda ; MD ; PhD ; ^{k-honda@tenor.ocn.ne.jp} ; ^{k-honda@md.tsukuba.ac.jp} (Forensic Scientist) ; Yukiko Sugano ; PhD (Medicine Historian) ; Shizue Yano ; MD (Forensic Pathologist) ; Takeki Nishi ; MS (Forensic Scientist) ; Yuko Sekine ; MD (Forensic Pathologist)
• 关键词：Forensic science ; STR ; Vanadium ; Personal identification ; Ancient DNA ; DNA typing
• 刊名：Journal of Forensic and Legal Medicine
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：20
• 期：4
• 页码：326-333
• 全文大小：1366 K

文摘

Polymerase chain reaction (PCR) has been rapidly established as one of the most widely used techniques in molecular biology. Because most DNA analysis is PCR-based, the analysis of unamplifiable DNA of poor quality or low quantity is nearly impossible. However, we observed that if an appropriate concentration of vanadium chloride is added to the standard reaction mixture, the enzymatic amplification of DNA could be enhanced. Using multiplex PCR with the addition of vanadium, DNA typing was possible from even trace amounts of DNA that we were unable to amplify using normal reaction conditions.

This method might be an effective tool for not only criminal investigations and ancient DNA analysis, but also for nearly all fields using DNA technology.