Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali

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• 作者：Zhongwei Wu ; Junwen Lu ; Xiaoqing Wang ; Bing Hu ; Hong Ye ; Jialong Fan ; Muhammad Abid ; Xiaoxiong Zeng
• 关键词：Paecilomyces hepiali HN1 ; Polysaccharides ; Optimization ; Antitumor activity
• 刊名：Carbohydrate Polymers
• 出版年：2 January, 2014
• 年：2014
• 卷：99
• 期：Complete
• 页码：226-234
• 全文大小：2437 K

文摘

In the present study, optimal medium for the growth of mycelia and the production of exopolysaccharides from Paecilomyces hepiali HN1 (PHEPS) in submerged culture was investigated. As a result, the maximum production of mycelia (12.98 卤 0.14 g/L) and PHEPS (5.33 卤 0.11 g/L) were achieved under the optimal medium of sucrose 46.08 g/L, yeast extract 4.71 g/L, (NH₄)₂SO₄ 5.72 g/L, KH₂PO₄ 1.70 g/L, CaCl₂ 0.50 g/L, MgSO₄ 0.50 g/L, potato extract 1% and malt extract 1%. Furthermore, the antitumor activity of PHEPS in vitro was evaluated by using three cell lines of human liver tumor HepG2 cells, breast cancer MCF-7 cells and cervical cancer Hela cells. It was found that PHEPS exhibited relative higher anti-proliferative activity against HepG2 cells than MCF-7 cells and Hela cells. At a concentration of 500 渭g/mL and 72 h treatment, the inhibition rate of PHEPS on HepG2 cells reached to 62.58%. All these results suggested that PHEPS could be explored as novel natural antitumor agent with great potential application.