Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis
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- 作者：Gwang Hyeon Eom ; Kabsun Kim ; Sung-Mi Kim ; Hae Jin Kee ; Ji-Young Kim ; Hye Mi Jin ; Ju-Ryoung Kim ; Jung Ha Kim ; Nakwon Choe ; Kee-Beom Kim ; Junwon Lee ; Hyun Kook ; Nacksung Kim ; Sang-Beom Seo
- 关键词：Histone ; Methyltransferase ; PRDM8 ; Steroidogenesis ; Transcription
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2009
- 出版时间：9 October 2009
- 年：2009
- 卷：388
- 期：1
- 页码：131-136
- 全文大小：697 K
文摘
A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.