Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia

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• 作者：Lei Feng^a ; Junyi Yin^a ; Shaoping Nie^a ; Yiqun Wan^a ; ^b ; ^{wanyiqun@ncu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Mingyong Xie^a ; ^{myxie@ncu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CP ; cassia polysaccharide ; CP-30 ; cassia polysaccharide with 30% (v/v) ethanol precipitation ; CP-40 ; cassia polysaccharide with 40% (v/v) ethanol precipitation ; Com-CG ; commercial cassia gum ; HPGPC ; high performance gel permeation chromatography ; FT-IR ; fourier transform infrared spectroscopy ; GC&ndash ; MS ; gas chromatography&ndash ; mass spectroscopy ; FITC ; fluorescein isothiocyanate ; LPS ; lipopolysaccharide
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：91
• 期：Complete
• 页码：946-953
• 全文大小：1179 K

文摘

The seeds of m>Cassia obtusifoliam> are widely used as a drink in Asia and an additive in food industry. Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities. In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained. Different rheological properties for CP-30 and CP-40 were found, indicating the differences in structure characteristics between CP-30 and CP-40. Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated. Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose. The immunomodulatory effects of CP, CP-30 and CP-40 were assessed. All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed. The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.