Existence of different types of senile plaques between brain and spinal cord of TgCRND8 mice

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• 作者：Qiuju Yuan^a ; Huanxing Su^f ; Yalun Zhang^c ; Wing Hin Chau^c ; Cheung Toa Ng^c ; Wutian Wu^b ; ^d ; ^e ; ^g ; ^{linzx@cuhk.edu.hk} ; Zhi-Xiu Lin^a ; ^{wtwu@hkucc.hku.hk}
• 关键词：Senile plaques ; Alzheimer&rsquo ; s disease ; Spinal cord and glial reaction ; TgCRND8 mice
• 刊名：Neurochemistry International
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：62
• 期：3
• 页码：211-220
• 全文大小：1915 K

文摘

Conflicting findings exist regarding the formation of diffuse and dense-core ¦Â-amyloid (A¦Â) plaques in Alzheimer¡¯s disease (AD). In the present study, we characterized A¦Â plaque types in the brain and spinal cord of TgCRND8 mice, which express a transgene incorporating both the Indiana mutation (V717F) and the Swedish mutations (K670N/M671L) in the human amyloid-¦Â protein precursor (APP) gene. By combining immunohistochemistry and thioflavin S staining, we were able to define dense-core and diffuse plaques in neocortex of the brain and spinal cord of 9 week-, 5 month-, 10 month- and 20-month-old TgCRND8 mice. The senile plaques in the neocortex were predominantly dense-core plaques, even in the youngest mice. However, diffuse plaques were instead detected in spinal cord of the mice, regardless of age. Our results that relative predominance of dense-core plaques in the neocortex and diffuse plaques in the spinal cord of TgCNRD8 mice of all disease durations argue against the notion that diffuse plaques may represent an early stage in the evolution of dense-core plaques. Furthermore, we also found that the ratio of A¦Â42/A¦Â40 of the brain was much higher than that of the spinal cord by A¦Â ELISA assay. Our findings strongly indicate that diffuse and dense-core plaques may form via independent processes in AD and A¦Â42 is more prone to form dense-core plaques than is A¦Â40.