Isolation of the Mouse (MFH-1) and Human (FKHL14) Mesenchyme Fork Head-1 Genes Reveals Conservation of Their Gene and Protein Structures

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• 作者：Miura ; Naoyuki ; Iida ; Kiyoshi ; Kakinuma ; Hideaki ; Yang ; Xiao-Li ; Sugiyama ; Toshihiro
• 刊名：Genomics
• 出版年：1997
• 出版时间：May 1, 1997
• 年：1997
• 卷：41
• 期：3
• 页码：489-492
• 全文大小：166 K

文摘

The very recently found evolutionarily conserved DNA-binding domain of 100 amino acids, termed the fork head domain, emerged from a sequence comparison of the rat hepatocyte transcription factor HNF-3α and the homeotic genefork headofDrosophila.We previously isolated a new member of this family, the mesenchyme fork head-1 (MFH-1) gene, which is expressed in developing mesenchyme. Here we describe the isolation of the mouse (MFH-1) and human (FKHL14) chromosomal MFH-1 genes and the determination of the gene and protein structures of MFH-1. We found that the MFH-1 gene has no introns and that the identity of the amino acid sequences of mouse and human MFH-1 proteins is 94 % . We also investigated the transcriptional activity of the mouse and human MFH-1 proteins and found that both proteins act as positive transactivators.