Nuclear magnetic resonance spectroscopy of 3β,7β-dihydroxy-5-cholen-24-oic acid multi-conjugates: unusual bile acid metabolites in human urine

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• 作者：Genta Kakiyama ; Shoujiro Ogawa ; Takashi Iida ; Yasuo Fujimoto ; Kumiko Mushiake ; Takaaki Goto ; Nariyasu Mano ; Junichi Goto and Toshio Nambara
• 关键词：¹H NMR ; ¹³C NMR ; Two-dimensional NMR ; Bile acid multi-conjugate ; Glycine-amidated bile acid ; Taurine-amidated bile acid ; Sulfated bile acid ; N-Acetylglucosaminidated bile acid
• 刊名：Chemistry and Physics of Lipids
• 出版年：2006
• 出版时间：April 2006
• 年：2006
• 卷：140
• 期：1-2
• 页码：48-54
• 全文大小：328 K

文摘

Complete ¹H and ¹³C resonance assignments were made for a new type of 3β,7β-dihydroxy-5-cholen-24-oic acid doubly conjugated with sulfuric acid at C-3 and N-acetylglucosamine at C-7 and its glycine- and taurine-amidated triple-conjugates by the combined use of several homonuclear and heteronuclear shift-correlated 2D NMR techniques. The effects of sulfation at C-3, N-acetylglucosaminidation at C-7, and aminoacyl amidation at C-24 on the ¹H and ¹³C chemical shifts and signal multiplicity were clarified. The shielding data serving to characterize each of the bile acid multi-conjugates are also discussed.