An experimental framework for improved selection of binding proteins using SNAP display

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• 作者：Gillian Houlihan^a ; ^b ; Pietro Gatti-Lafranconi^a ; Miriam Kaltenbach^a ; David Lowe^b ; Florian Hollfelder^a ; ^{fh111@cam.ac.uk" class="auth_mail}
• 关键词：Protein engineering ; Therapeutic protein ; SNAP display ; Directed evolution ; In vitro compartmentalization ; In vitro selection
• 刊名：Journal of Immunological Methods
• 出版年：March, 2014
• 年：2014
• 卷：405
• 期：Complete
• 页码：47-56
• 全文大小：778 K

文摘

Display technologies (e.g. phage and ribosome display) are powerful tools for selecting and evolving protein binders against various target molecules. SNAP display is a DNA display technology that is conducted entirely in vitro: DNA encoding a library of variants is encapsulated in water-in-oil droplets wherein in vitro protein expression and covalent coupling to the encoding DNA occurs. Here, we explore critical factors for the successful performance of SNAP display based on a set of experiments designed to measure and quantify to what extent they affect selection efficiency. We find that, in SNAP display, the reconstituted cell free expression system PURExpress led to 1.5-fold more active protein and achieved 3.5-fold greater DNA recovery in model selections compared to the RTS 100 Escherichia coli lysate based expression system. We report on the influence parameters including droplet occupancy, valency and selection stringency have on recovery and enrichment. An improved procedure involving bivalent display and stringent selection against a model target, Her2, led to a 10⁷-fold enrichment of a DARPin (H10-2-G3, known to bind Her2 with picomolar affinity) over a non-binding DARPin after three rounds of selection. Furthermore, when spiked into a mixture of DARPins with different affinities, DARPin H10-2-G3 outcompeted all other variants demonstrating SNAP display's ability to efficiently resolve clones with affinities in the nano- to picomolar range. These data establish SNAP display as an in vitro protein engineering tool for isolating protein binders and provide a framework for troubleshooting affinity selections.