In vitro biosynthesis and genotoxicity bioassay of silver nanoparticles using plants

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• 作者：Kamal K. Panda^a ; V. Mohan M. Achary^a ; R. Krishnaveni^a ; Bijaya K. Padhi^b ; Sachindra N. Sarangi^c ; Surendra N. Sahu^c ; Brahma B. Panda^a ; ^{panda.brahma@gmail.com"" rel=""nofollow}
• 关键词：Silver nanoparticles ; Pandanus odorifer ; Reactive oxygen species ; Allium genotoxicity bioassay ; Oxidative stress
• 刊名：Toxicology in Vitro
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：25
• 期：5
• 页码：1097-1105
• 全文大小：900 K

文摘

Silver nanoparticles (AgNP-P) from AgNO₃ were synthesized by using the broth prepared from the aromatic spath of male inflorescence of screw pine, Pandanus odorifer (Forssk.) Kuntze AgNP-P was then characterized by UV–visible spectroscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS). Functional groups in the broth were analyzed by Fourier Transform infrared spectroscopy (FTIR). Genotoxicity of AgNP-P was assessed by utilizing our well-established Allium cepa assay system with biomarkers including the generation reactive oxygen species (ROS: and H₂O₂), cell death, mitotic index, micronucleus, mitotic aberrations; and DNA damage by Comet assay. Other chemical forms of silver such as Ag⁺ ion, colloidal AgCl, and AgNP-S at doses 0–80 mg L⁻¹ were included for comparison with AgNP-P. The results revealed that AgNP-P and AgNP-S exhibited similar biological effects in causing lesser extent of cytotoxicity and greater extent of genotoxicity than that was exhibited by Ag⁺ ion alone. Among different tested chemical forms of silver, colloidal AgCl was identified to be the least cytotoxic and genotoxic. Cell death and DNA-damage induced by AgNP-P were prevented by Tiron and dimethyl thiourea that scavenge and H₂O₂, respectively. The present findings demonstrated the role of ROS in the AgNP-induced cell death and DNA damage.