Characterization of CD8⁺ leukocytes in fugu (Takifugu rubripes) with antiserum against fugu CD8α

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• 作者：Kyosuke Araki ; Kanako Akatsu ; Hiroaki Suetake ; Kiyoshi Kikuchi ; Yuzuru Suzuki
• 关键词：CD8 ; T cells ; Lymphocytes ; Antiserum ; Flow cytometry ; Mitogen ; Fugu ; Takifugu rubripes
• 刊名：Developmental and Comparative Immunology
• 出版年：2008
• 出版时间：2008
• 年：2008
• 卷：32
• 期：7
• 页码：850-858
• 全文大小：955 K

文摘

We have investigated the characteristics of CD8⁺ leukocytes by using an anti-CD8 antiserum raised in mouse by DNA-immunization. The magnetically sorted CD8⁺ peripheral blood leukocyte (PBL) population comprised lymphocytes/thrombocytes and monocytes, whereas CD8⁻ PBLs consisted of lymphocytes/thrombocytes, monocytes, and neutrophils. Expression analysis demonstrated that both groups of cells expressed the CD3ε and TCR genes. The CD8 and CD8β genes were detected only in CD8⁺ cells, whereas expression of CD4 and immunoglobulin light chain (IgL) was observed only in CD8⁻ cells. These results suggest that fugu CD8⁺ leukocytes contain CD8⁺ T cells, but not CD4⁺ T cells or B cells. Furthermore, mitogenesis of the CD8⁺ lymphocyte/thrombocyte population was induced by phytohemaglutinin stimulation, suggesting that fish CD8⁺ lymphocytes/thrombocytes (probably CD8⁺ T cells) have characteristics similar to mammalian CD8⁺ T cells. Neutrophils and monocytes/macrophages infiltrating a subcutaneous inflammatory site expressed only CD8, but not CD8β, CD4, TCR, or IgL. This result suggests that similar to mammalian dendritic cells, fugu monocytes/macrophages express CD8.