Expression profiles of peroxiredoxin proteins of the rodent malaria parasite Plasmodium yoelii

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• 作者：Kawazu ; Shin-ichiro ; Nozaki ; Tomoyoshi ; Tsuboi ; Takafumi ; Nakano ; Yoko ; Komaki-Yasuda ; Kanako ; et. al.
• 关键词：Antioxidant ; Insect stage ; Malaria ; Peroxiredoxin ; Plasmodium yoelii
• 刊名：International Journal for Parasitology
• 出版年：2003
• 出版时间：November, 2003
• 年：2003
• 卷：33
• 期：13
• 页码：1455-1461
• 全文大小：276 K

文摘

Patterns of expression of the 2-Cys and 1-Cys peroxiredoxin (Prx) proteins of the rodent malaria parasite Plasmodium yoelii during its life cycle were observed by immunofluorescent antibody staining and confocal laser scanning microscopy. 2-Cys Prx was expressed in the parasite cytoplasm throughout the life cycle, and the thioredoxin (Trx)-peroxidase activity of 2-Cys Prx revealed with the recombinant protein suggested that the Prx is constitutively expressed and, thus, likely plays a housekeeping role in the parasite's intracellular redox control. In contrast, 1-Cys Prx showed stage-specific expression in blood-stage parasites. The limited expression of 1-Cys Prx in the trophozoite cytoplasm suggests that 1-Cys Prx may be involved in haemoglobin metabolism by the parasite, which generates a prooxidative haem iron and increases intracellular oxidative stress. The antioxidant activity of 1-Cys Prx was tested for its ability to protect yeast enolase against inactivation of the mixed-function oxidation system. Differential expression of the two Prx proteins during the erythrocytic and insect stages suggests the importance of these proteins in protecting parasites against oxidative stress, which is generated by the parasite's metabolism and also from the environment.