Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine

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• 作者：Kohji Seio ; ^{kseio@bio.titech.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Yurie Ohno ; Kentaro Ohno ; Leo Takeshita ; Takashi Kanamori ; Yoshiaki Masaki ; Mitsuo Sekine
• 关键词：Photo-cage ; DNA-protein interaction ; MutS
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：1 October 2016
• 年：2016
• 卷：26
• 期：19
• 页码：4861-4863
• 全文大小：511 K

文摘

Mismatch binding protein MutS binding to bulge structure in DNA duplexes was controlled by UV irradiation. 4-O-(2-Nitrobenzyl)thymidine or 4-O-[2-(2-nitrophenyl)propyl]thymidine was incorporated into DNA duplexes a bulged position. The MutS did not bind to the caged DNA duplexes but bound after removing the 2-nitrobenzyl or 2-(2-nitrophenyl)propyl group by photo-irradiation. By using photo-caged DNA duplex, we revealed that binding of MutS to the uncaged DNA downstream of the T7 RNA promoter weakly inhibited transcription by T7 RNA polymerase.